Example:

Document doc = new Document();
DocumentBuilder builder = new DocumentBuilder(doc);

Shape shape = builder.insertChart(ChartType.SCATTER, 432.0, 252.0);
Chart chart = shape.getChart();

// Delete default generated series.
chart.getSeries().clear();
ChartSeries series = chart.getSeries().add("AW Series 1", new double[] { 0.7, 1.8, 2.6, 3.9 },
        new double[] { 2.7, 3.2, 0.8, 1.7 });

// Set marker formatting.
series.getMarker().setSize(40);
series.getMarker().setSymbol(MarkerSymbol.SQUARE);
ChartDataPointCollection dataPoints = series.getDataPoints();
dataPoints.get(0).getMarker().getFormat().getFill().presetTextured(PresetTexture.DENIM);
dataPoints.get(0).getMarker().getFormat().getStroke().setForeColor(Color.YELLOW);
dataPoints.get(0).getMarker().getFormat().getStroke().setBackColor(Color.RED);
dataPoints.get(1).getMarker().getFormat().getFill().presetTextured(PresetTexture.WATER_DROPLETS);
dataPoints.get(1).getMarker().getFormat().getStroke().setForeColor(Color.YELLOW);
dataPoints.get(1).getMarker().getFormat().getStroke().setVisible(false);
dataPoints.get(2).getMarker().getFormat().getFill().presetTextured(PresetTexture.GREEN_MARBLE);
dataPoints.get(2).getMarker().getFormat().getStroke().setForeColor(Color.YELLOW);
dataPoints.get(3).getMarker().getFormat().getFill().presetTextured(PresetTexture.OAK);
dataPoints.get(3).getMarker().getFormat().getStroke().setForeColor(Color.YELLOW);
dataPoints.get(3).getMarker().getFormat().getStroke().setTransparency(0.5);

doc.save(getArtifactsDir() + "Charts.MarkerFormatting.docx");